The advantages of the ultra-clean table are that it is easy to operate, relatively comfortable, high in work efficiency, short in preparation time, and can be operated more than 10 minutes after starting up, and can basically be used at any time. In factory production, the vaccination workload is very large, and when it is necessary to work for a long time, the ultra-clean table is an ideal equipment. The ultra-clean table is powered by a three-phase motor with a power of about 145 to 260W. The air is blown out through a "super filter" composed of special microporous foam plastic sheets laminated to form a continuous dust-free The ultra-clean laminar flow of bacteria, so-called "efficient special air", removes dust, fungi, bacterial spores, etc. larger than 0.3 μm. The flow rate of ultra-clean air is 24 ～ 30m / min, which is enough to prevent the pollution caused by the possible disturbance of nearby air. Such a flow rate will not prevent the use of alcohol lamps or Bunsen burners to burn and disinfect instruments. The staff operates under such sterile conditions to keep the sterile materials free from contamination during the transfer inoculation process. However, in the event of a power outage in the middle of the operation, materials exposed to unfiltered air will not be spared pollution. At this time, the work should be completed quickly, and a mark should be made on the bottle. If the material in it is in the multiplication stage, it will no longer be used for multiplication and transferred to rooting culture in the future. If it is a general production material, it can be discarded because it is extremely rich. If it is in the rooting process, it can be reserved for future planting.
The ultra-clean platform power supply mostly adopts three-phase four-wire system, one of which has a neutral wire, which is connected to the machine casing, and should be firmly connected to the ground wire. The other three wires are all phase wires, and the working voltage is 380V. There is a certain sequence in the three-wire access circuit. If the wire end is connected incorrectly, the wind will reverse. At this time, the sound is normal or slightly abnormal. There is no wind on the front of the ultra-clean platform (the alcohol lamp flame can be used to observe the movement and should not be tried for a long time). Cut off the power supply in time, as long as the wire ends of any two phases are exchanged and then connected, it can be solved. If the three-phase line is only connected to two phases, or one of the three phases is in poor contact, the machine sound is very abnormal, and you should immediately cut off the power supply and carefully repair it, otherwise it will burn the motor. These common senses should be clearly explained to the staff when starting to use the ultra-clean platform to avoid accidents and losses that should not be caused.
The air inlet of the ultra-clean platform is under the back or front. There is a common foam plastic sheet or non-woven fabric in the metal mesh cover to block large particles of dust. It should be checked and dismantled and washed frequently. If the foam plastic is found to be aging, it should be replaced in time. In addition to the air inlet, if there are air leakage holes, they should be plugged tightly, such as sticking adhesive tape, stuffing cotton, sticking glue paper, etc. Inside the metal mesh cover on the front of the workbench is a super filter. The super filter can also be replaced. If the dust particles are clogged due to age and the wind speed is reduced, as long as the aseptic operation cannot be guaranteed, you can replace it with a new one.
The service life of the ultra-clean table is related to the cleanliness of the air. In the temperate zone, the ultra-clean bench can be used in general laboratories. However, in tropical or sub-tropical areas, because the atmosphere contains high amounts of pollen or dusty areas, the ultra-clean bench should be placed in a better indoor room with double doors use. Under no circumstances should the air inlet hood of the ultra-clean platform be placed against an open door or window, so as not to affect the service life of the filter.
The sterile room should be regularly sprayed with 70% alcohol or 0.5% phenol for dust reduction and disinfection. Use 2% Xin Jie Er to wipe off the countertops and utensils (70% alcohol is also available), use formalin (40% formaldehyde) plus a small amount of permanganic acid Potassium is regularly sealed and fumigated, combined with ultraviolet sterilization lamps (more than 15 minutes each time) and other disinfection and sterilization methods, so that the sterile room often maintains a high degree of sterility. The inside of the inoculation box should also be equipped with an ultraviolet lamp. Turn on the lamp for more than 15 minutes before use to irradiate the sterilization, but there are bacteria in the place where the irradiation is not possible. When the ultraviolet lamp is turned on for a long time, it can excite oxygen molecules in the air to form ozone molecules. This gas component has a strong sterilization effect and can produce a sterilization effect on the corners where the ultraviolet rays do not directly shine. Because ozone is harmful to health, the ultraviolet lamp should be turned off before entering the operation, and you can enter it more than ten minutes after turning off.
Ultraviolet lamps can also be installed on the ultra-clean workbench, but they should be installed outside the lampshade, and the lamps should be staggered and arranged in parallel, so as not to hinder the lighting during work. If the UV lamp is installed in the lampshade (glass plate), this is useless, because the UV cannot penetrate the glass, and its lamp tube is made of quartz glass, not silicate glass.
The inoculation room strives for simplicity, and all items that are not directly related to the work of this room should not be placed in order to maintain sterility. The air in the inoculation room should be absolutely isolated from the outside air, and the reserved ventilation holes should be sealed as far as possible. The ventilation holes can be equipped with upper and lower air windows. The area of ​​the air window should be slightly larger. It needs to be covered with 4 layers of gauze for simple dust filtering. After a day's work, you can open the window to fully ventilate, and then close it. In short, both clean and dust-free and sterile, and fresh air, suitable for work. The gauze covering the ventilation window should be changed frequently. However, the above-mentioned measures are only ideal design schemes, which are often difficult to achieve in an all-round way. In fact, as long as strict aseptic procedures are followed, the room with open doors and windows is protected by an ultra-clean platform, and the pollution rate of inoculation can still be controlled in production. Level.
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