Summary:
The two most commonly used methods for analyzing real-time quantitative PCR experimental data are absolute and relative quantification. Absolute quantification uses the standard curve to calculate the copy number of the starting template; the relative quantification method compares the expression difference between the target transcripts of the treated and untreated samples. 2ï¼ â–³â–³ CT method is a convenient method for analyzing relative changes of gene expression in real-time quantitative PCR experiment, that is, a convenient method for relative quantification. This paper introduces the derivation, assumptions and application of this method. In addition, in this paper we also introduce the derivation and application of two 2- △△ CT derivation methods, which may be used in real-time quantitative PCR data analysis.
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