At the beginning of the month, our company launched a new batch of ELISA kits, of which the hyaluronic acid kit is the main product of this month, and the quality is also absolutely guaranteed. If you provide free testing services, please call Manager He if you need to order! We made a special share for our friends. Let's take a look at the three methods of extracting hyaluronic acid.
1. Animal Organization
The main raw materials are cockscomb and bull's eye vitreous. Degrease and dehydrate the raw materials with acetone or ethanol, soak and filter with distilled water, then treat with sodium chloride aqueous solution and chloroform solution, then add trypsin to keep the mixture, and finally use ion exchange agent for treatment and purification to obtain refined and transparent Quality acid.
2. Microbial fermentation
Fermentation broth with glucose as carbon source. Fermentation in the medium for 48 hours. After the fermentation is completed, the mycelium and impurities are removed by filtration, and then a high-purity product is obtained by a simple operation such as alcohol precipitation. The advantage of hyaluronic acid produced by fermentation is that the molecular weight can be set according to the product design. The key to the fermentation method lies in the choice of strains, mostly streptococcus, lactococci, etc.
3. Chemical synthesis
A natural enzyme polymerization reaction is used; first, a polysaccharide polymer is used to synthesize "hyaluronic acid oxacyclopentene derivative", then a hydrolytic enzyme is added to produce a derivative and enzyme complex, and finally a reaction at 90 degrees Celsius Hyaluronic acid is synthesized by removing enzymes from the liquid. The artificial synthesis method can greatly reduce the manufacturing cost of hyaluronic acid, but the structure is less refined.
The above are the three methods for extracting hyaluronic acid. Shanghai Hengyuan ELISA kits are complete. If there are quality problems, we will return them unconditionally. If you have any questions, please contact us.
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