This reagent is intended for research use only and is designed to quantify the levels of amyloid beta 1-40 (Aβ1-40) in rat serum, plasma, and other biological liquid samples. The assay is based on the double-antibody sandwich immunoassay principle. A microtiter plate is pre-coated with a specific monoclonal antibody against rat Aβ1-40, which acts as a solid-phase capture antibody. After incubation with the sample, the target Aβ1-40 binds to the immobilized antibody. Subsequently, a horseradish peroxidase (HRP)-labeled secondary antibody is added, forming a complex of antibody-antigen-enzyme-labeled antibody. Following thorough washing to remove unbound components, the TMB substrate is introduced. Under the catalytic action of HRP, TMB turns blue and then changes to yellow when an acidic stop solution is added. The intensity of the color developed is directly proportional to the concentration of Aβ1-40 in the sample. The optical density (OD) is measured at 450 nm using a microplate reader, and the concentration of Aβ1-40 in the sample is determined by comparing it to a standard curve generated from known concentrations.
The kit includes all necessary reagents for performing the assay. It is available in both 48-well and 96-well configurations. The components include:
- Coated microplate: 1 × 48 or 1 × 96 wells
- Standard: 0.5 mL × 1 bottle (900 μg/L)
- Standard Diluent: 1.5 mL × 1 bottle
- Enzyme Conjugate: 3 mL × 1 bottle (for 48-well) or 6 mL × 1 bottle (for 96-well)
- Sample Diluent: 3 mL × 1 bottle (for 48-well) or 6 mL × 1 bottle (for 96-well)
- TMB Developer A: 3 mL × 1 bottle (for 48-well) or 6 mL × 1 bottle (for 96-well)
- TMB Developer B: 3 mL × 1 bottle (for 48-well) or 6 mL × 1 bottle (for 96-well)
- Wash Buffer (20×): 20 mL × 20 times (for 48-well) or 20 mL × 30 times (for 96-well)
- Sealing Film: 2 pieces (for 48-well) or 2 pieces (for 96-well)
- Sealing Bag: 1 piece
Storage conditions: All components should be stored at 2–8°C. The coated plate should be kept sealed and protected from light. Proper storage ensures stability and performance of the reagents. This kit provides a reliable and sensitive method for quantifying Aβ1-40 in rat biological samples, making it suitable for various research applications related to neurodegenerative diseases such as Alzheimer’s.
Long Pants
long jeans pants for men,men's long pants,mens linen long pant,men's printed joggers cotton long pants
Guangdong Metagarden Trading Co.,Ltd , https://www.smtfurniture.com