Elisa kit specification for human exein elisa kit Specifications: 48-well configuration / 96-well configuration Standard dilution: 1.5ml × 1 bottle of enzyme label reagent: 3 ml × 1 bottle (48) / 6 ml × 1 Bottle (96) [human exein elisa kit] This reagent is for research use only: the concentration of the standard is used as the abscissa, the OD value is the ordinate, and a standard curve is drawn on the coordinate paper, according to the sample The corresponding concentration of the OD value is found by the standard curve; then multiplied by the dilution factor; or the linear regression equation of the standard curve is calculated from the concentration of the standard and the OD value, and the OD value of the sample is substituted into the equation to calculate the sample concentration, and then Multiplied by the dilution factor is the actual concentration of the sample. Kit composition: sealing film: 2 pieces (48) / 2 pieces (96) Instructions: 1 sealed bag: 1 standard product: 2700ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle at 2-8 ℃ to store enzyme Standard coated plate: 1 × 48 1 × 96 2-8 ° C Store sample dilution: 3ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Store Developer A solution: 3ml × 1 bottle 6 ml × 1 bottle 2 -8 ℃ Storage Developer B solution: 3ml × 1 bottle 6 ml × 1 bottle 2-8 ℃ Storage stop solution: 3ml × 1 bottle 6ml × 1 bottle 2-8 ℃ Storage concentrated washing solution: (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle at 2-8 ° C. Experimental principle: This kit uses the double antibody sandwich method to determine the level of human extein in the specimen. Microporous plates were coated with purified human extein antibody to make solid phase antibodies. Exein was added to the monoclonal antibody-coated microwells in turn, followed by HRP-labeled exonin (extein) ) The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with extein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human extein in the sample was calculated by a standard curve. Purpose: This kit is used to determine the content of extein in human serum, plasma and related liquid samples. Service commitment: Delivery period: payment to delivery. Free technical consultation and guidance during working hours. Please call to provide customers with sample testing services to maximize the validity of the experimental results (free substitute testing). Storage conditions and expiration date: kit storage: 2-8 ℃ | expiration date: 6 months
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