Note: This measurement method is demonstrated using the analysis of a butanol isomer mixture. The procedure includes instrument and reagent preparation.
1. Instrumentation: A gas chromatograph, a DNP column (as prepared in section 5.3.2), a hydrogen gas cylinder, and a set of reagent bottles are required for this experiment.
2. Reagents: Isobutanol, sec-butanol, tert-butanol, and n-butanol (all of AR grade) are needed.
Next, prepare the mixed sample. Take a clean and dry vial, such as a penicillin bottle, and accurately weigh the following amounts: 0.4 g of tert-butanol, 0.5 g of sec-butanol, 0.7 g of isobutanol, and 0.9 g of n-butanol. Ensure that the weights are measured to the third decimal place for precision. After weighing, mix the components thoroughly by shaking or stirring until a homogeneous mixture is obtained. Once prepared, store the sample in a sealed container and allow it to rest before injection into the gas chromatograph. This step ensures accurate and reproducible results during the chromatographic analysis.
This detailed preparation helps minimize variability and ensures reliable identification and quantification of each butanol isomer in the mixture.
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