elisa principle
The basis of ELISA is the immobilization of antigen or antigen and enzyme labeling of antigen or. The antigen bound on the surface of the solid phase carrier may still maintain its immunological activity. The enzyme-labeled antigen or antibody retains both its immunological activity and enzyme activity. During the measurement, the test specimen (the antibody or antigen in it) is reacted with the antigen or antibody on the surface of the solid phase carrier. The antigen-antibody complex formed on the solid phase carrier is separated from other substances in the liquid by washing. Enzyme-labeled antigens or antibodies are added, and are also bound to the solid-phase carrier through the reaction. At this time, the amount of enzyme on the solid phase is proportional to the amount of test substance in the specimen. After adding the substrate for the enzyme reaction, the substrate is catalyzed by the enzyme into a colored product. The amount of the product is directly related to the amount of the test substance in the specimen, so qualitative or quantitative analysis can be performed according to the depth of the color. Due to the high catalytic efficiency of the enzyme, the results of the immune response are indirectly amplified, making the assay method highly sensitive.
2. Types of ELISA
ELISA can be used to measure antigens and antibodies. There are three necessary in this measurement method: (1) solid phase probiotic or antibody, namely "immunosorbent"; (2) enzyme-labeled antigen or antibody, called "conjugate" ); (3) Substrate for enzyme reaction. According to the source and the situation of the specimen and the specific conditions of the test, various types of test methods can be designed
Shanghai Hengyuan Biological Technology Co., Ltd. is an agent specializing in immunology, cytology, and chemical reagents. Now our company can represent American ATCC cells, American Sciencell cells, American CHI cells, colleagues. Our company also has a complete range of passage cells, all The cells are accompanied by passage and culture instructions. Our company is now cooperating with American companies and accepts custom-made primary cells from customers. It is limited to human, mouse and rat normal tissues and tumor tissue cells. The minimum number of cells is 1 million. Customers who are in need are welcome to come to consult.
The company has long-term operation of scientific research elisa kits, and the agent brands are well-known brands such as American R & D, IBL, bendor, etc. At the same time, the company also produces various types of elisa kits, which use imported reagents. The experimental effect is comparable to the original import. The company also provides testing services for customers and provides technical guidance. Customers are welcome to come to consult.
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