The Composition of Media
Medium and Ingredients
1. Acetobacter Medium
Glucose (glucose) 100g, Yeast extract (yeast extract) 10g, CaCO3 20g, Agar (agar) 15g, Distilled water 1000ml, Adjust pH to 6.8.
Application: Odorous variant of Acetobacter aceti.
2. Nutrient Agar (Nutrition Gravy Agar)
Peptone 5g, Beef extract 30g, NaCl 5g, Agar (agar) 15g, Distilled water, Adjust pH to 7.0-7.2.
Note: When cultivating Bacillus, add 5mg of MnSO4·H2O to promote spore formation.
Application: Gas-producing bacteria, Alcaligenes faecalis, Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and many others.
3. Corn Meal Medium
Maize flour 5g, Peptone 0.1g, Glucose (glucose) 1g, Tap water 1000ml.
Note: Boil the mixture in an autoclave at 121°C for 1 hour, then distribute into 18x18 mm tubes with 10 ml each. Sterilize again at 121°C for 1 hour.
4. Lactic-bacteria Medium I (Lactic Acid Bacteria Medium I)
Yeast extract 7.5g, Peptone 7.5g, Glucose (glucose) 10g, KH2PO4 2g, Tomato juice 100ml, Tween 80 0.5ml, Distilled water 900ml, pH 7.0.
Application: Lactobacillus plantarum, thermophilic lactic acid streptococci.
5. Lactic-bacteria Medium II (Lactic Acid Bacteria Medium II)
Lacto-casein peptone 10g, Beef extract 10g, Yeast extract 5g, Glucose (glucose) 5g, Tween 80 1g, K2HPO4 2g, Na-acetate 5g, Diamine citrate 2g, MgSO4·7H2O 0.2g, MnSO4·H2O 0.05g, Distilled water 1000ml, pH 6.5–6.8.
Application: Lactobacillus plantarum.
6. Peptone Glucose Yeast extract Medium PGY
Peptone 10g, Yeast extract 5g, Glucose (glucose) 1g, Distilled water 1L.
7. Glycerol Agar
Peptone 5g, Beef extract 3g, Glycerol (Glycerin) 20g, Tap water 1000ml, Agar (agar) 15g, pH 7.0–7.2.
8. Rhizobium medium AS 9
Yeast extract 1g, Soil extract 200ml, Mannitol 10g, Agar (agar) 15g, Distilled water 800ml, pH 7.2.
Note: Prepare soil extract by suspending 50g dried garden soil in 200ml tap water, boil for 15 hours, filter, and adjust volume to 200ml.
Application: Soybean rhizobium, cowpea rhizobium, peanut rhizobium, etc.
9. Mannitol Agar
Yeast extract 5g, Peptone 3g, Mannitol 25g, Agar (agar) 15g, Distilled water 1000ml.
10. Glucose Asparagine Agar
Glucose (glucose) 10g, Asparagine (asparagine) 0.5g, K2HPO4 0.5g, Water 1000ml, pH 7.2–7.4.
Application: Pseudomonas aeruginosa, purple micromonospora.
11. Gause’s Synthetic Agar
KNO3 1g, Soluble starch 20g, K2HPO4 0.5g, MgSO4·7H2O 0.5g, NaCl 0.5g, FeSO4 0.01g, Agar (agar) 20g, Water 1000ml, pH 7.2–7.4.
Application: Pseudomonas aeruginosa, Streptomyces species.
12. Wort Agar
Dilute wort (without hops) to 12 Brix, add 15g agar per 1000ml, melt by heating, and dispense into tubes. Autoclave at 110°C for 30 minutes.
Application: Candida species, Saccharomyces cerevisiae, Pichia, Rhodotorula, and many others.
13. Potato Dextrose Agar (PDA)
Potato extract 1000ml, Dextrose (glucose) 20g, Agar (agar) 20g.
Note: Prepare potato extract by boiling 200g peeled potatoes in 1000ml water for 30 minutes, filter, and add glucose and agar.
Application: Aspergillus, Fusarium, Penicillium, Trichoderma, and more.
14. Czapek’s Agar
Sucrose (sucrose) 30g, NaNO3 3g, MgSO4·7H2O 0.5g, KCl 0.5g, FeSO4·7H2O 0.01g, K2HPO4 1g, Agar (agar) 13g, Distilled water 1000ml, pH 7.2.
Application: Aspergillus, Penicillium, Streptomyces species.
15. Concentrated Sucrose Czapek’s Agar
Sucrose (sucrose) 200g, NaNO3 3g, MgSO4·7H2O 0.5g, KCl 0.5g, FeSO4·4H2O 0.01g, K2HPO4 1g, Agar (agar) 13g, Distilled water 1000ml.
Application: Aspergillus species.
16. Synthetic Potato Medium
20% Potato extract 1L, Glucose (glucose) 20g, KH2PO4 3g, MgSO4·7H2O 1.5g, Thiamine (Vitamin B1) trace, Agar (agar) 15g, pH 6.
Application: Aspergillus, Beauveria, Fusarium, Trichoderma, and more.
17. Corn Meal Medium
Mix corn meal with water [20:100(W/V)], distribute into 100ml bottles with ~30ml each, sterilize at 121°C for 30 minutes.
18. Filter Paper Medium
(NH4)2SO4 1g, KH2PO4 1g, MgSO4·7H2O 0.7g, NaCl 0.5g, Distilled water 1000ml, one 6x1cm filter paper strip.
19. Soybean Cake Meal Agar
10% infusion of soybean cake meal (10% soy cake powder dip), 1000ml, Glucose (glucose) 10g, NaCl 2.5g.
20. Sawdust, Wheat-bran Medium
Sawdust of broad leaf tree 75g, wheat bran 25g. Mix well, add appropriate water, fill into bottles, plug with cotton, and sterilize at 15 lbs for 1 hour.
21. Pine Block or Pine Sawdust Medium
Immerse 10x2x1 cm pine strips in 1-2% sucrose solution, allow them to absorb, fill into bottles, wrap in paper, and sterilize at 20 lbs for 1 hour.
22. Rice Straw. Dung Medium
Dried horse dung 65%, dried straw 35%, Urea 0.5%, Calcium superphosphate 0.7%, Gypsum 1%, Ammonium sulfate 0.3%. Add appropriate water, pile, and ferment.
23. Straw Rice Bran Medium
Dried straw 75–80%, rice bran 20–25%. Select non-moldy straw, cut into 3 cm pieces, soak in fresh water for 12 hours, add rice bran, and sterilize at 15 lbs for 1 hour.
24. Rice Medium
Fill 10g rice into a 50ml Erlenmeyer flask, add 20–30ml water, sterilize at 121°C for 30 minutes. Alternatively, 2g rice in 15x150mm tube with 6ml water, sterilize at 121°C for 30 minutes.
25. Filter Paper Medium
(NH4)2SO4 1g, KH2PO4 1g, MgSO4·7H2O 0.5g, Yeast Extract 0.1g, Distilled water 1000ml, 1x7 cm filter paper strip.
Note: Distribute 1ml of broth into each test tube, sterilize, and add a sterilized filter paper strip aseptically.
26. Yeast Extract-Malt Extract Agar I
Yeast extract 10g, Malt extract 10g, Glucose (glucose) 4g, Distilled water 1000ml, Agar (agar) 20g, pH 7.0.
Application: Streptomyces erythromycin.
27. Yeast Extract Peptone Agar
Yeast extract 1g, Multi-peptone 2g, Beef extract 1g, Glucose 10g, Agar (agar) 20g, Distilled water 1L, pH 7.0.
28. Glucose Yeast Extract Starch Agar
Glucose (glucose) 10g, Yeast extract (yeast extract) 10g, Starch (starch) 10g, NaCl 5g, CaCO3 3g, Agar (agar) 20g, Distilled water 1L, pH 7.0.
29. Wheat Bran Medium
Wheat bran (bran) 36g, (NH4)2HPO4 10g, K2HPO4 0.2g, MgSO4·7H2O 0.1g, Agar (agar) 20g, Distilled water 1L, pH 7.0.
30. Yeast Sporulation Medium
(1) Kleyn Medium: KH2PO4 0.012%, K2HPO4 0.02%, Sodium acetate 0.5%, Glucose 0.062%, NaCl 0.062%, Peptone 0.25%, Biotin 2μg%, Agar 2%, Trace element solution 1ml/100ml.
(2) Gorodkowa Medium: Glucose 0.1%, Peptone 1%, NaCl 0.5%, Agar 2%.
(3) McClary Medium: Glucose 0.1%, KCl 0.18%, Yeast juice 0.25%, Sodium acetate 0.82%, Agar 1.5%.
31. BPY Medium
Beef extract 5g, Peptone 10g, NaCl 5g, Yeast extract 5g, Glucose (glucose) 5g, Agar (agar) 1–2%, Distilled water 1000ml, pH 7.0.
Application: Escherichia coli, Pseudomonas putida.
32. LB Medium
Yeast extract 5g, Peptone 10g, NaCl 10g, Agar (agar) 1–2%, Distilled water 1000ml, pH 7.0.
Application: Escherichia coli.
33. Penssay Broth
Beef extract 1.5g, Yeast extract 1.5g, Tryptone 5g, NaCl 3.5g, Glucose (glucose) 1g, K2HPO4·3H2O 4.8g, Distilled water 1000ml, pH 7.2.
34. Blood Agar
Peptone 10g, Glucose 2g, NaCl 5g, NaH2PO4 2.5g, Sodium citrate 5g, Brain infusion 800ml, Pig heart infusion 200ml, 10% blood, pH 7.2.
Note: Prepare pig brain and pig heart infusions by boiling, filtering, and mixing.
35. Cellulose-decomposing Bacteria Synthetic Medium
NaCl 6g, MgSO4·7H2O 0.1g, KH2PO4 0.5g, CaCl2 0.1g, K2HPO4 2.0g, Yeast extract 1.0g, (NH4)2SO4 2.0g, Distilled water 1000ml, Filter paper strip, pH 7.0–7.4.
Note: Solid medium for cellulose-decomposing bacteria: Carboxymethyl cellulose 2%, Agar 2%.
36. Clostridium Pasteurianum Synthetic Medium
Glucose (glucose) 1%, MnSO4 0.001%, KH2PO4 0.05%, FeSO4 0.001%, K2HPO4 0.05%, Yeast extract 0.1%, MgSO4·7H2O 0.02%, Peptone 0.01%, NaCl 0.001%, CaCO3 0.5%, Distilled water 100%, pH 7.0.
37. Yeast Extract-Malt Extract Agar II
Yeast extract 4g, Malt extract 10g, Glucose (glucose) 4g, Agar (agar) 17g, Distilled water 1000ml, pH 7.3.
38. Oatmeal Agar
Oatmeal (infusion) 20g, Agar (agar) 18%, Trace element solution 1ml, Distilled water to 1000ml, pH 7.2.
Note: Trace element solution: FeSO4·7H2O 0.1g, MnCl2·4H2O 0.1g, ZnSO4·7H2O 0.1g, Distilled water 100ml.
39. Calcium Malate Agar
Calcium malate 10g, K2HPO4 0.5g, NH4Cl 0.5g, Glycerol (glycerol) 10g, Agar (agar) 15g, Distilled water 1000ml.
40. Thiobacillus Ferrooxidans Medium
(NH4)2SO4 0.15g, KH2PO4 0.05g, KCl 0.05g, MgSO4·7H2O 0.5g, Ca(NO3)2·4H2O 0.01g, FeSO4·7H2O 50g, Distilled water 1000ml, pH 2.0.
41. Thiobacillus Thiooxidans Medium
(NH4)2SO4 0.3g, KH2PO4 3–4g, CaCl2 (anhydrous) 0.25g, MgSO4·7H2O 0.5g, FeSO4·7H2O 0.001g, Distilled water 1000ml, pH 3.5–4.
42. Plasma Substitute Medium
Sucrose (sucrose) 12.5–15%, Peptone (peptone) 0.5%, KH2PO4 0.03%, Na2HPO4·12H2O 0.14%, pH 7.0–7.2.
43. Skim Milk Medium ATCC 183
Skim milk 100g, deionized water 1000ml.
Application: Lactobacillus bulgaricus, Streptococcus thermophilus.
44. Pea Agar
Glucose (glucose) 1%, Peptone (peptone) 0.5%, NaCl 0.5%, Agar (agar) 2.0%, Pea infusion 12–14 mg/100ml, pH 7.2–7.5 before sterilization.
Note: Prepare pea infusion by boiling peas, adjusting pH, adding chloroform, and sterilizing.
45. Standard Quality of Pea Infusion: Aminonitrogen 160–200 mg/100ml, Soluble phosphorus 400–600 μg/ml, Color: golden, transparent.
Sit Stand Desk,Sit and Stand Desk,Electric Sit Stand Desk,Stand Up Sit Down Desk,Best Sit Stand Desk
Suzhou Uplift Intelligent Technology Co., Ltd , https://www.upliftec.com