After receiving the ELISA kit, teachers should ensure proper storage according to the label instructions. Before use, allow the kit to reach room temperature. Also, remember that the diluted standards must be discarded and not saved, as improper storage can affect experimental results.
1. Substrate A is volatile, so avoid leaving the lid open for extended periods. Substrate B is light-sensitive, so keep it away from direct light. Read the OD values immediately after completing the experiment to ensure accuracy.
2. Unused reagents should be properly sealed or covered. Never mix reagents from different batches, as this may lead to inconsistent results.
3. Always use disposable tips to prevent cross-contamination. When handling stop solution, substrate A, and substrate B, avoid using pipettes with metal parts, as they may react with certain reagents.
4. When washing the microplate, make sure to pat it dry thoroughly. Do not insert absorbent paper directly into the wells, as this can cause contamination or damage to the plate.
5. Maintain a consistent order when adding reagents to ensure all wells have the same incubation time, which is critical for accurate results.
6. Follow the incubation times, volume of liquid added, and the sequence specified in the instruction manual precisely. Deviating from these steps may lead to unreliable data.
7. Any unused strips should be returned to the sealed bag immediately to protect them from degradation and maintain their integrity.
8. Use clean plastic containers to prepare the wash solution. Ensure all components and samples are well mixed before use to guarantee uniformity.
Zhengzhou Weier Biotechnology Co., Ltd. is a comprehensive enterprise integrating research, production, and sales. The company offers a wide range of biological agents, chemical reagents, preclinical testing materials, ELISA kits, antibodies, and standards. We also provide culture media and other life science products. With a complete product line and reliable quality, we welcome both new and existing customers to inquire about our services. Let us support your research and experiments with professional solutions.
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