The working principle of the biological safety cabinet is to maintain the negative pressure inside the safety cabinet, let the airflow enter the safety cabinet from the outside, and the pathogenic microorganisms in the tissue cabinet overflow, thereby protecting the experimental operator, and the circulating air and the exhaust air are filtered by the high efficiency filter. It also protects the product and the laboratory environment. It is not difficult to find that the filter is a relatively important part of the protection of the operator protection, product protection and cross-contamination after replacing the high-efficiency filter.
Maintenance and replacement of biosafety cabinet filters High efficiency filters have a long life. How to determine that a high-efficiency filter needs to be replaced: a. As the life cycle increases, dust and bacteria accumulate in the filter, resulting in an increase in high-efficiency filter pressure loss. It is hoped that the user can operate the equipment in the background environment of clean air. If it is operated in a poor air environment for a long time, it will lead to frequent replacement of the high efficiency filter. During the operation of the equipment, if the equipment emits an audible and visual alarm and is caused by a low flow rate or a low flow rate of the inflow airflow (see the display window respectively indicating that the value is lower than the design requirement), the filter plugging is very serious, and the fan speed has reached the maximum. Value, the system can not reach the specified wind speed value anyway, the filter must be replaced.
b. Check the cumulative running time of the fan on the control panel. If it reaches 10000 hours, it is usually necessary to replace the filter, but it is not necessary to replace it. Also consider the cleanliness of the environment, usage habits, etc. It is recommended to use a method to determine whether it needs to be replaced. Device.
The biosafety cabinet filter replacement method is as follows:
1. Turn off the power switch to cut off the power cable of the safety cabinet.
2. Pull out the wiring of the front hanging door and the box, and remove the front hanging door and lay it flat.
3. After the operator has taken protective measures, remove the cover of the high-efficiency filter mounting hole.
4. Remove the high-efficiency filter press plate. Remove the high-efficiency filter, and at the same time put the waste high-efficiency filter into the prepared plastic bag and seal the mouth to be treated.
5. Install the new high-efficiency filter as required, restore the accessories removed before the door, install the hanging door and restore the power connection.
6. After the power is turned on, it is necessary to check the filter, and it can be used only after it meets the requirements.
7. The above work should be completed by professionals.
Special Note: During the use of the safety cabinet, care should be taken not to place soft, subtle items (such as soft tissue, tampon, etc.) on the work surface to prevent it from being inhaled by the return air grille on the table. In the air duct and fan, it affects the operation of the equipment.
Check daily to ensure that the seal between the filter and the system is secure and leak free. In the event of a leak, apply a wrench to tighten the screw cap. Since the filter may be filled with biohazardous materials, it should be replaced or repaired by a professional. It is necessary to carry out the safety cabinet before replacement and/or repair. Disinfect and clean.
Before the high-efficiency filter is replaced, the inner surface of the cabinet and the original ultra-high efficiency filter must be sterilized by formaldehyde. When replacing, it is best to let the used laboratory technician present (pre-inquiry about the bacteria used in the experiment). Persons with injuries to the hands and body should not operate. Operators must wear gas masks and rubber gloves. The removed filter should be sealed immediately into a plastic bag and placed in a carton. The used gloves and gloves are also treated and burned together. When installing a new filter, do not put your head and body into the cabinet, and behave with great care.
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