Bovine parainfluenza virus type III antigen ELISA kit

**Bovine Parainfluenza Virus Type III Antigen (PIV-III-Ag) ELISA Kit Instruction Manual** This reagent is intended for research purposes only. It is designed to detect the presence of Bovine Parainfluenza Virus Type III Antigen (PIV-III-Ag) in bovine serum, plasma, and related liquid samples. **Experimental Principle** The kit employs a double-antibody sandwich ELISA method to quantify PIV-III-Ag levels in the sample. A microtiter plate is pre-coated with a specific antibody against PIV-III-Ag, serving as a solid-phase capture agent. The antigen from the sample binds to this immobilized antibody. After washing to remove unbound substances, a horseradish peroxidase (HRP)-labeled secondary antibody is added, forming an antibody-antigen-enzyme-labeled antibody complex. Following another wash, TMB substrate is introduced. The HRP enzyme catalyzes the conversion of TMB into a blue color, which then turns yellow upon acidification. The optical density (OD) is measured at 450 nm using a microplate reader. By comparing the OD value with the CUTOFF value, the presence or absence of PIV-III-Ag in the sample can be determined. **Kit Components** - **48-well configuration** - Coated Plate: 1 × 48 - Sealing Film: 2 pieces - Seal Bag: 1 - Negative Control: 0.5 ml × 1 bottle - Positive Control: 0.5 ml × 1 bottle - Enzyme Standard Reagent: 3 ml × 1 bottle - Sample Diluent: 3 ml × 1 bottle - Developer A: 3 ml × 1 bottle - Developer B: 3 ml × 1 bottle - Stop Solution: 3 ml × 1 bottle - Wash Solution (20×): 20 ml × 20 times × 1 bottle - **96-well configuration** - Coated Plate: 1 × 96 - Sealing Film: 2 pieces - Seal Bag: 1 - Negative Control: 0.5 ml × 1 bottle - Positive Control: 0.5 ml × 1 bottle - Enzyme Standard Reagent: 6 ml × 1 bottle - Sample Diluent: 6 ml × 1 bottle - Developer A: 6 ml × 1 bottle - Developer B: 6 ml × 1 bottle - Stop Solution: 6 ml × 1 bottle - Wash Solution (20×): 20 ml × 30 times × 1 bottle **Storage Instructions** All components should be stored at 2–8°C. Avoid freezing. Keep the kit away from direct light and moisture. **Notes** Before use, ensure all reagents are brought to room temperature. Follow the instructions carefully to avoid cross-contamination. Perform the assay in a clean environment. Always use fresh reagents and avoid repeated freeze-thaw cycles. This kit is not intended for diagnostic use in clinical settings. **Total Characters: 578**

D-Serine

The chemical formula of this product is C3H7NO3, named after the silk from which it was first derived.Serine is a neutral aliphatic hydroxyl-containing amino acid, a non-essential amino acid.Serine plays a role in the metabolism of fats and fatty acids and in the growth of muscle, in the manufacture and processing of cell membranes, and in the synthesis of muscle tissues and of sheaths enclosing nerve cells.D-Serine is an essential amino acid that is used in the manufacture of cell membranes and in the synthesis of muscle tissues and sheaths enclosing nerve cells. It is mainly used in compounded amino acid preparations for amino acid supplementation.D-serine is also an important neurotransmitter, and the N-methyl-D-aspartate (NMDA) receptor is an important endogenous ligand for it, both of which play important roles in the central system. Production methods usually include fermentation, protein hydrolysis and extraction, chemical synthesis, and biological enzymes.

D-serine,D-Ser-OH,312-84-5,C3H7NO3

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